Cytoplasmic Male Sterility-Associated Mitochondrial Gene orf312 Derived from Rice (Oryza sativa L.) Cultivar Tadukan

Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) restorer (TAR) were obtained successive backcrossing Oryza sativa cultivars Tadukan (a cytoplasmic donor) Taichung 65 recurrent parent). Using Illumina HiSeq, we determined whole-genome sequences of mitochondria TAA screened genome for presence open reading frame ( orf ) genes specific to this genome. One these genes, orf312 , showed differential expression patterns in TAR anthers at meiotic mature stages, transcript amounts being less than those TAA. The gene similar previously described orf288 part which among components comprising WA352 chimeric CMS-associated wild-abortive-type CMS. Conclusions promising candidate